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KMID : 1098420050130010048
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Korean Journal of Medicinal Crop Science
2005 Volume.13 No. 1 p.48 ~ p.51
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Karyotype Analysis and Physical Mapping of rDNAs Using McFISH in Jeffersonia dubia Benth
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Kim Soo-Young
Choi Hae-Woon
Koo Dal-Hoe
Kim Chan-Soo
Bang Jae-Wook
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Abstract
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Karyotype analysis and chromosomal locailization of 45S and 5S rDNAs using McFISH (multi-color fluorescence in situ hybridization) were carried out in Jeffersonia dubia Benth., which is one of medicinal plants belonging to Berberidaceae. The somatic metaphase chromosome number was 2n=2x=12 and the size of chromosomes ranged 1.95{sim}3.50;{mu}m. The chromosome complement consisted of two pairs of metacentrics (chromosomes 1 and 3), two pairs of submetacentrics (chromosomes 2 and 4) and two pairs of subtelocentrics (chromosomes 5 and 6). In McFISH, one pair of 45S rDNA site was detected on the centromeric region of chromosome 2 and three pairs of 5S rDNA sites were detected on the short arm of chromosomes 4, 5 and 6, respectively.
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KEYWORD
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Jeffersonia dubia, karyotype, rDNAs, McFISH
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